Description
Lipases and Phospholipases
Methods and Protocols
Sandoval, Georgina (Ed. / Hrsg.)
ISBN 978-1-0716-5471-2 · Humana · XII, 340 p. 52 illus., 51 illus. in color. · Third Edition 2027
Description / Beschreibung
This third edition volume expands on the previous editions with an update on phospholipases, including structure-function relationships using the new tools of artificial intelligence, as well as current and new applications of both enzymes. The chapters in this book are organized into three parts: Part One provides readers with an overview on lipases and phospholipases; Part Two covers quantitative methods for assessing lipase activity and case studies on cloning and comparison of host for lipase expression; and Part Three presents relevant applications of lipases in new emerging fields such as nanobiocatalysis, polymers synthesis/degradation, and biosensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough, Lipases and Phospholipases: Methods and Protocols, Third Edition is a practical and detailed reference for both graduate students and expert scientists. This book will be useful to the scientific community working with lipases, phospholipases, and related enzymes.
Contents / Inhaltsverzeichnis
Lipases: An Overview.- Phospholipases: Where Do We Stand?.- Quantitative Methods for Assessing True Lipase Activity Using Long-Chain Triglycerides and Complex Lipid Substrates.- Cloning and Protein Expression of CpEst in Komagataella phaffii.- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.- Lipase Activity, Analytical Methods: Brief Review and Gold Standards.- Phospholipases D: An Update on Structure – Function Relationships.- Synthesis of Esters of 5-Hydroxymethylfurfural Catalyzed by Lipases in a Continuous Packed-Bed Bioreactor.- Synthesis and Purification of Lipase-Catalyzed Sugar E.- Lipase-Catalyzed Synthesis of Nutritional Structured Lipids at Laboratory-Scale Using Crude of Refined Oils.- Lipase-Catalyzed Pathways to the Synthesis of Pharmacologically Active Amides.- Lipases and Cutinases from Phytopathogenic and Saprobic Fungi in Plastic Depolymerization and Synthesis: A Review.- Biopolymers Derived from Solanum lycopersicum: Synthesis with CAL-B and Their Structural Analysis by Mass Spectrometry (DI-ESI and MALDI-TOF).- Functionalization of Flavonoids with Lipases.- Two-Step Lipase-Catalyzed Acylation of Non-Glycosylated Flavonoids: Application to Phloretin.- Assessment of the Hypoglycemic and Hypolipidemic Activities of Fructans and Their Laurate Bioconjugates.- Bio-Inspired Silica Lipase Nanobiocatalysts for the Synthesis of Fatty Acid Methyl Esters.- Identification of Domain Formation during sPLA2 Activity by Biophotonics.- Lipase/Esterase Activity as an Indicator of Milk Spoilage Process: A Sequential Injection Analysis Methodology.
Features / Besonderheiten
Include cutting-edge methods and protocol Provides step-by-step detail essential for reproducibl results Contains key notes and implementation advice from the experts
