Description
Ultrastructural Neuroscience Methods
Tremblay, Marie-Ève (Ed. / Hrsg.)
ISBN 978-1-0716-5455-2 · Humana · X, 266 p. 67 illus., 55 illus. in color. With online files/update. ·
Description / Beschreibung
This volume explores the study of the brain ultrastructure using various methodological approaches from different perspectives. The chapters in this book cover topics such as biological sample preparation, transmission and scanning electron microscopy imaging, and cutting-edge qualitative and quantitative analysis. The chapters provide essential considerations and protocols for the study of animal models, human cellular models, and human post-mortem brain samples across contexts of health and disease. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Comprehensive and thorough, Ultrastructural Neuroscience Methods is a key resource for biomedical, translational, and clinical researchers interested in delving into the mysteries of the brain at a nanoscale resolution.
Contents / Inhaltsverzeichnis
Freeze-Fracture Electron Microscopy Methods for Evaluation of Nerve Cells and Synapses.- How to Combine Light and Electron Microscopy to Detect Lipids.- Immunoelectron Microscopy of Brain Receptors: Focus on G Protein-Coupled Receptors.- An Alternative Chromogenic Immunostaining Method for Light and Electron Microscopy: A Demonstration Using NeuN Staining in Mouse Brain Samples.- Mapping Mitochondrial Architecture in the Brain: Methods and Insights.- Ultrastructure of Glial Cells in iPSC-Derived Unguided Neural Organoids from APOE4/4 Patients with Alzheimer’s Disease.- A Guide for Microglial Ultrastructural Analysis.- Reproducible Quantification Method to Differentiate Dark vs Intermediate vs Typical Appearance of Immune Cells in Electron Microscopy A Priori, Including Dark Microglia.- The Dynamic Side of Electron Microscopy: How to Use Quantitative Electron Microscopy to Portray the Gold Particle’s Dance Style.- Multimodal Imaging of Breast-to-Brain-Metastatic Lesions.- SnapG: An Automated Tool for Myelin g-ratio Measurement with Batch Processing Capability.- Ultrastructural Volumetry of Spinal Cord White Matter Mitochondria via 3D Reconstruction from Conventional Transmission Electron Microscopy.- Postmortem Human Brain Tissue Processing for Transmission Electron Microscopy of Gray Matter Myelin.
Features / Besonderheiten
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
